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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Enrichment of superoxide dismutase 2 in glioblastoma confers to acquisition of temozolomide resistance that is associated with tumor-initiating cell subsets

Fig. 5

SOD2 modulation affected drug susceptibility and TIC features. a The frequency of the TIC-featured population in U87MG or A172 resistant cells was estimated using the in vitro extreme limiting dilution assay. b The cells were transfected with SOD2 siRNA and the indicated proteins were detected by western blotting. c Detection of CD133 level in SOD2-overexpressing U87MG or A172 parental cells was done with Western blotting. (*P < 0.05) (d) The SOD2 expression of the resistant primary tumor cells (GBM#1) transfected by shRNA lentiviral vector was detected with Western blotting (left upper panel). The spheroid assay was applied with the control and the knockdown cells cultured in 0.3% methylcellulose and the serum-free medium until formation of the sphere (The scale bar is 1000 μm). The diameter and the number of the spheroid cells were calculated in the bar graphs. e The frequency of the TIC-featured population of GBM#1 was estimated using the in vitro extreme limiting dilution assay. f The indicated protein levels in GBM#1 cells (left) and the CD133+ subsets (right) transfected with SOD2 siRNA were detected with Western blotting

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