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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Glucosamine impedes transforming growth factor β1-mediated corneal fibroblast differentiation by targeting Krüppel-like factor 4

Fig. 2

Effects of GlcN on TGF-β1-induced α-SMA expression in HCFs. (a and b) Immunoblots showing the expression levels of KLF4 and α-SMA in HCFs after application of the indicated concentration of GlcN for 24 h (a) or 48 h (b), with or without 10 ng/ml TGF-β1. ACTN served as a loading control. Asterisk indicated a statistically significant difference (p < 0.05) between groups. Each value represented the mean ± SD for three experiments. (c) Photomicrographs showing the immunofluorescent staining of KLF4 and α-SMA in HCFs after application of the indicated concentration of GlcN for 24 h, with or without 10 ng/ml TGF-β1

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