Fig. 6From: Glucosamine impedes transforming growth factor β1-mediated corneal fibroblast differentiation by targeting Krüppel-like factor 4Effects of GlcN on the stability of KLF4 protein in HCFs. (a and b) Immunoblots showing levels of KLF4 in HCFs after treatment with vehicle (a) or 5 mM GlcN (b) plus 50 ng/ml CHX for the indicated times. ACTN served as a loading control. (c and d) Graphic representations of KLF4 protein levels derived from western analyses like those depicted in panels A and B. Shown are the relative fold and percentage changes in the presence of CHX. Bars and symbols depict the mean ± SD (n = 3)Back to article page