Fig. 1
From: Yy1 regulates Senp1 contributing to AMPA receptor GluR1 expression following neuronal depolarization
Membrane depolarization represses the SUMO protease Senp1. (a) The relative mRNA levels of Senp1 in cortical neurons was determined by qRT-PCR following treatment with vehicle (Control), 25 mM potassium chloride (KCl) for 2 hours (hrs), 25 mM KCl for 24 hrs, 60 mM KCl for 2 hrs, or 60 mM KCl for 24 hrs. Graphs indicate three independent biological replicates. Error bars represent one standard deviation from the mean. *(p < 0.05). p value was determined using two-tailed unpaired t test. (b) Western blot analysis of Senp1 expression level following neuronal depolarization in cortical neurons. Total proteins were extracted from cortical neurons after 2 hrs treatment with 60 mM KCl and control treatment. Actin was used as loading control.