Fig. 6

Hypoxia in EV-A71-infected muscle was rescued by timely treatment with IFNαA. a A schematic diagram of mouse IFNαA treatment after EV-A71 infection. Six different experimental groups of mice received mIFNαA (300 IU/g of mouse) at different dpi and frequencies of treatments. Therapeutic efficacies were assayed by survival rates. In some cases, infected and treated mice (n = 12) were monitored for up to 1.5 years. b Clinical scores were significantly reduced only in Exp. I (green) and Exp. II (red), when treated at dpi 1, or dpi 1, 2, 3. c Survival rates of Exp. I (green) (100%) and Exp. II (red) (88%) were significantly higher than that of the control group with no IFNαA treatment. d No VP1-positive signal was detected in the muscle by IHC at 25 dpi and 41 dpi in mice recovered from IFNαA treatment in Exp. 1. e The myogenin protein (black arrowhead) was expressed in recovered muscle via IHC staining, indicating muscle regeneration after injury. f HIF1A and VEGFA proteins were both expressed in newly recovered muscle (dpi = 25), but not in the fully recovered muscle (dpi = 41)