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Table 1 The primer sequences, annealing temperature and fragment sizes for molecular analysis

From: Analysis of polymorphisms, promoter methylation, and mRNA expression profile of maternal and placental P53 and P21 genes in preeclamptic and normotensive pregnant women

 

Method

Primer Sequence 5′ ➔ 3’

PCR Product (bp)

Tm °C

 P21-rs1059234

PCR-RFLPa

Forward: CCCAGGGAAGGGTGTCCTG

Reverse: GGGCGGCCAGGGTATGTAC

298

64

 P21-rs1801270

PCR-RFLP

Forward: GTCAGAACCGGCTGGGGATG

Reverse: CTCCTCCCAACTCATCCCGG

272

64

 TP53-rs1042522

PCR-RFLP

Forward: GTCCCAAGCAATGGATGAT

Reverse: CAAAAGCCAAGGAATACACG

551

61

 P21

promoter region

MSPb

FM: TACGCGAGGTTTCGGGATC

RM: CCCTAATATACAACCGCCCCG

174

60

FU: GGATTGGTTGGTTTGTTGGAATTT

RU: ACAACCCTAATATACAACCACCCCA

164

 P53

promoter region

MSP

FM: GTAGTTTGAACGTTTTTATTTTGGC

RM: CCTACTACGCCCTCTACAAACG

115

60

FU: GTAGTTTGAATGTTTTTATTTTGGT

RU: CCTACTACACCCTCTACAAACA

115

 P21

Real-time PCR

Forward: GCTCTGCTGCAGGGGACAGC

Reverse: TCTGCCGCCGTTTTCGACCC

136

60

 P53

Real-time PCR

Forward: GAGCTGAATGAGGCCTTGGA

Reverse: CTGAGTCAGGCCCTTCTGTCTT

151

60

 β-Actin

Real-time PCR

Forward: CCTGGCACCCAGCACAAT

Reverse: GCCGATCCACACGGAGTACT

70

60

  1. a Polymerase chain reaction–restriction fragment length polymorphism b Methylation Specific PCR