| Method | Primer Sequence 5′ ➔ 3’ | PCR Product (bp) | Tm °C |
---|---|---|---|---|
 P21-rs1059234 | PCR-RFLPa | Forward: CCCAGGGAAGGGTGTCCTG Reverse: GGGCGGCCAGGGTATGTAC | 298 | 64 |
 P21-rs1801270 | PCR-RFLP | Forward: GTCAGAACCGGCTGGGGATG Reverse: CTCCTCCCAACTCATCCCGG | 272 | 64 |
 TP53-rs1042522 | PCR-RFLP | Forward: GTCCCAAGCAATGGATGAT Reverse: CAAAAGCCAAGGAATACACG | 551 | 61 |
 P21 promoter region | MSPb | FM: TACGCGAGGTTTCGGGATC RM: CCCTAATATACAACCGCCCCG | 174 | 60 |
FU: GGATTGGTTGGTTTGTTGGAATTT RU: ACAACCCTAATATACAACCACCCCA | 164 | |||
 P53 promoter region | MSP | FM: GTAGTTTGAACGTTTTTATTTTGGC RM: CCTACTACGCCCTCTACAAACG | 115 | 60 |
FU: GTAGTTTGAATGTTTTTATTTTGGT RU: CCTACTACACCCTCTACAAACA | 115 | |||
 P21 | Real-time PCR | Forward: GCTCTGCTGCAGGGGACAGC Reverse: TCTGCCGCCGTTTTCGACCC | 136 | 60 |
 P53 | Real-time PCR | Forward: GAGCTGAATGAGGCCTTGGA Reverse: CTGAGTCAGGCCCTTCTGTCTT | 151 | 60 |
 β-Actin | Real-time PCR | Forward: CCTGGCACCCAGCACAAT Reverse: GCCGATCCACACGGAGTACT | 70 | 60 |