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Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Neuropeptide S-initiated sequential cascade mediated by OX1, NK1, mGlu5 and CB1 receptors: a pivotal role in stress-induced analgesia

Fig. 7

Restraint stress increases the NPS level in the LH (a) and the substance P level in the vlPAG. (b) Brain tissues containing the LH or vlPAG were punched and homogenized from restrained mice immediately after a 30 min-restraint stress (stress group) or from unrestrained control mice (non-stress group). NPS levels in LH homogenates were measured by an ELISA kit (Cusabio, College Park, MD, USA), whereas substance P level in vlPAG homogenates were measured by an EIA kit (Caymon Chemical. Ann Arbor, MI, USA). *p < 0.05, **p < 0.01 vs. the Non-stress control group (Student’s t-test)

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