Fig. 4

HK2 is required for collagen XVII-mediated increases of glycolysis and CSC features in lung cancer cells. a and b, PCR array showed increased expression of HK2 in both cells cultured in spheroid medium and cells with collagen XVII overexpression, indicating the HK2 plays an important role in collagen XVII-induced metabolic reprogramming in lung cancer cells. c, RT-PCR and western blot analysisshowed increased HK2 expression in both cells cultured in spheroid medium and cells with collagen XVII overexpression. d, RT-PCR and western blot analysis showed decreased expression of HK2 in cells with collagen XVII knockdown. e, Glycolysis was decreased in collagen XVII overexpressed lung cancer cells with HK2 knockdown. f, The glucose uptake and lactate level were decreased in collagen XVII overexpressed lung cancer cells with HK2 knockdown. g, However, OCR was not significantly different between collagen XVII overexpressed lung cancer cells with or without HK2 knockdown. h, The relative ATP content and the percentage of mitotracker red staining were neither different between collagen XVII overexpressed lung cancer cells with or without HK2 knockdown. i, The number of sphere formation decreased in collagen XVII overexpressed lung cancer cells with HK2 knockdown. j, Collagen XVII overexpressed lung cancer cells with HK2 knockdown were more sensitive to cisplatin. k, The capability of tumor initiation was decreased in collagen XVII overexpressed lung cancer cells with HK2 knockdown