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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

Fig. 2

The effects of circ_0003204 on HAEC proliferation, motility and tube formation. a QRT-PCR analysis of the transfection efficiency of circ_0003204 overexpression or si-circ_0003204 after transfection for 48 h in HAECs. b QRT-PCR analysis of USP36 mRNA level normalised to GAPDH after transfection with circ_0003204 siRNA or negative control into HAECs for 48 h. c and d Cellular viability analysis of HAECs transfected with circ_0003204 overexpression vector or circ_0003204 siRNA using CCK-8 assay. e Analysis of cell migration potential of HAECs transfected with circ_0003204 overexpression or si-circ_0003204 vectors using transwell assay. Scale bar = 25 μm. f Wound healing assay for analysis of ox-LDL-exposed HAECs migration after transfection of circ_0003204 overexpression or si-circ_0003204 vectors. Scale bar = 50 μm. g Analysis of vasculogenesis ability of HAECs transfected with circ_0003204 overexpression or si-circ_0003204 vectors. Scale bar = 100 μm. OE, overexpression; siRNA, small interfering RNA; NC, negative control; CTL, control. Data are the means ± SD of three experiments. *P and **P < 0.05

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