Skip to main content

Advertisement

Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

Fig. 3

Circ_0003204 serves as a sponge for miR-370 in HAECs. a A microarray heat map from GSE13139 representing discrepant mRNA expression values in HAECs treated by ox-LDL (50μg/ml) for 24 h compared with those treated by control (fold change > 2, P< 0.05). b The standardization of differently expressed mRNAs in GSE13139. c A volcano plot presenting the differently expressed mRNAs (|log2FC| > 2, P < 0.05) from GSE13139. d A scatter plot assessing mRNA expression variation between the sham and ox-LDL-treated group from GSE13139. e Schematic illustration showing the overlap between the predicted miRNAs for circ_0003204 by Circinteractome and the predicted miRNAs for upregulated DEG using the combination of Targetscan, miRanda and miRDB. f Schematic illustration showing the overlap between the predicted miRNAs for circ_0003204 using Circinteractome and the predicted miRNAs for significantly upregulated DEGs from GSE28829 using Targetscan, miRanda and miRDB. g Schematic illustration showing the overlap between the predicted miRNAs for circ_0003204 using Circinteractome and the significantly downregulated miRNAs in GSE34645. h Schematic representation of two complementary binding sites of circ_0003204 with miR-370. i Luciferase activity of cotransfection of circ_0003204 and miR-370 mimics/negative control. g RIP indicated the correlation of circ_0003204 and miR-370 with Ago2. Ago2 protein was tested by IP-western blot, and the expression of circ_0003204 and miR-370 were investigated using qRT-PCR. k FISH assay showed the location of circ_0003204 and miR-370 in HAECs. Red, circ_0003204; Green, miR-370; Blue, DAPI. Scale bar = 20um. l QRT-PCR analysis showing the effect of ox-LDL on miR-370 level in HAECs. m MiR-370 expression in ox-LDL-treated HAECs measured by qRT-PCR after circ_0003204 overexpression or knockdown. n Circ_0003204 expression in ox-LDL-treated HAECs measured by qRT-PCR after miR-370 overexpression or knockdown. NC, negative controk; WT, wild type; MT, mutant; DAPI, 4′,6-diamidino-2-phenylindole; FISH, fluorescence in situ hybridisation; CTL, control; OE, overexpression; siRNA, small interfering RNA. Data are means ± SD of three experiments. *P and **P < 0.05.

Back to article page