Fig. 4

TGFβR2 is the downstream target of miR-370. a and c QRT-PCR analysis of TGFβR2 expression after transfection of miR-370 mimics or inhibitor to ox-LDL-treated HAECs for 48 h. b and d Western blot analysis of TGFβR2 protein expression after transfection of miR-370 mimics or inhibitor to ox-LDL-treated HAECs for 48 h. e Putative miR-370 binding sites in TGFβR2. The predictive complementary sequences are indicated in red. f Relative luciferase activity of wild-type and 3’UTR mutant constructs of TGFβR2 cotransfected with miR-370 mimics and miRNA negative control. NC, negative control; UTR, untranslated regions. Data are means ± SD of three experiments. *P < 0.05