Fig. 5
The circ_0003204/miR-370 axis could induce HAEC deactivation via its downstream gene TGFβR2. a Cell proliferation activity was measured by CCK-8 assay after transfection with TGFβR2 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05 versus siRNA control cotransfected with inhibitor control group. b Cell motility was measured by transwell assay after transfection with TGFβR2 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05. Scale bar = 25 μm. c Cell migration potential was detected by wound healing assay after transfection with TGFβR2 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05. Scale bar =50 μm. d Tube formation was measured by Matrigel assay after transfection with TGFβR2 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05. Scale bar = 100 μm. e Western blot analysis indicating phosph-SMAD3 and SMAD3 protein level after transfection with TGFβR2 siRNA into ox-LDL-treated HAECs. *P < 0.05. f Cell proliferation activity was measured by CCK-8 assay after SIS3 (3 μM) + ox-LDL (50 μg/ml) treatment. *P < 0.05. g Cell motility was measured by transwell assay after SIS3 (3 μM) + ox-LDL (50 μg/ml) treatment. *P < 0.05. Scale bar = 25 μm. h Cell migration was measured by wound healing assay after SIS3 (3 μM) + ox-LDL (50 μg/ml) treatment. *P < 0.05. Scale bar =50 μm. i Tube formation was measured by Matrigel assay after SIS3 (3 μM) + ox-LDL (50 μg/ml) treatment. *P < 0.05. Scale bar = 100 μm. j Western blot analysis showing TGFβR2 and phosph-SMAD3 expression after cotransfection of circ_0003204 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P and **P < 0.05. k Cell proliferation activity was measured by CCK-8 assay after transfection with circ_0003204 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05 versus group with contransfection of circRNA-siRNA NC and miRNA-inhibitor NC. l Cell motility was measured by transwell assay after transfection with circ_0003204 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05. Scale bar = 25 um. m Cell migration potential was detected by wound healing assay after transfection with circ_0003204 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05. Scale bar =50 μm. n Tube formation was measured by Matrigel assay after transfection with circ_0003204 siRNA + miR-370 inhibitor into ox-LDL-treated HAECs. *P < 0.05. Scale bar = 100 um. NC, negative control; siRNA, small interfering RNA. Data are means±SD of three experiments