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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: Circular RNA circ_0003204 inhibits proliferation, migration and tube formation of endothelial cell in atherosclerosis via miR-370-3p/TGFβR2/phosph-SMAD3 axis

Fig. 6

Expression level of circ_0003204 and miR-370 in EVs for the cerebral atherosclerosis patients and healthy controls. a The size distribution of isolated EVs measured by nanoparticle tracking analysis. b Immunoblotting of EV markers using anti-CD63 and anti-FLOT-1 and β-actin as a loading control. c The circ_0003204 expression in EVs for cerebral atherosclerosis patients and healthy control as measured by qRT-PCR. *P < 0.05. d The expression level of miR-370 in EVs from cerebral atherosclerosis patients and healthy control. e Size distribution histogram of cell medium EVs by nanoparticle tracking analysis. f Immunoblotting of cell medium EV for CD63, TSG101 and β-actin. g QRT-PCR analysis of circ_0003204 expression in the cell medium EVs at the different concentration of ox-LDL (0–100 μg/ml). *P < 0.05. h QRT-PCR analysis of circ_0003204 expression in the EVs collected from HAECs with ox-LDL treatment (50 μg/ml) at the indicated time points. *P < 0.05. Data are means ± SD of three experiments

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