Fig. 7
Effect of G-CSF protein on Behavioral test demonstrated in the a) Corner Test behavioral assay; b) locomotor activity test. a, b Percentage asymmetry is observed as a mouse enters a 300 corner and either turns left or right. In the above graph a, b percent asymmetry is compared before and after BCAO for sham, BCAO/Vehicle, and BCAO/G-CSF (n = 6). It is observed that mice treated with G-CSF after BCAO had a significant better percent asymmetry than those of BCAO/Vehicle and like the percent asymmetry of Sham mice on day 4 (F (5, 82) = 17.69, p < 0.0001) and day 7 (F (5, 32) = 5.902, p = 0.0006). c, d Average results (n = 6) of Corner test densitometric scanning are presented. (All data are presented as mean +/− SEM, #/* p < 0.05. # statistical significance between groups Sham and BCAO + Vehicle; *statistical significance between groups BCAO + Vehicle and BCAO + G-CSF). e, f Neuroprotection effect of G-CSF protein therapy in BCAO stroke mouse model using locomotor activity test. Locomotor activity of BCAO mice with and without G-CSF treatment were done after 4 days. Results show increased amount of activity with administration of G-CSF on day 4 (F (1.179, 12.97) = 26.12, p = 0.0001) and day 7 (F (1.259, 13.85) = 105.1, p < 0.0001). g, h Average results (n = 5) of locomotor test densitometric scanning are presented. The summary of locomotor data is provided in expressed in mean +/− SEM where n = 5 for Sham, BCAO and BCAO + G-CSF. a, c, e and g represent day 4; b, d, f and h represent day 7. Data shows animals treated with G-CSF have activity consistent with sham animals