Fig. 2

Competitive blocking assay for anti-Dectin-2 monoclonal antibodies in Dectin-2.Fc fusion protein to house dust mite, (a) The house dust mite Dermatophagoides pteronyssinus was coated and placed on an ELISA plate; then, the anti-Dectin-2 MoAbs 6A4G7 and 17A1D10 were added to compete the binding between D. pteronyssinus and Dectin-2.Fc. b and c Dectin-2.Fc fusion protein (10 μg/mL) was incubated on either the non-coated plates or the D. pteronyssinus-coated plates in the presence or absence of the anti-Dectin-2 MoAb 6A4G7 (b) or 17A1D10 (c) and IgG2a. HRP-conjugated goat anti-mouse MoAb was added, and absorbance at 450 nm was measured using a spectrophotometer. In non-coated plates (white column), binding of Dectin-2.Fc with any of the MoAbs was not observed. Conversely, Dectin-2.Fc was found to bind to D. pteronyssinus-coated plates (black column) in the presence and absence of IgG2a. Student’s t-test was used to compare MoAb treatment with the control IgG2a. * p < 0.05