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Table 3 Inhibition of IL-5 and IL-13 production in monocyte-derived dendritic cells/CD14 peripheral blood mononuclear cell co-culture in HDM stimulation by anti-Dectin 2 monoclonal antibody in patients with asthma

From: Antibody blockade of Dectin-2 suppresses house dust mite-induced Th2 cytokine production in dendritic cell- and monocyte-depleted peripheral blood mononuclear cell co-cultures from asthma patients

Anti-Dectin-2 MoAb6A4G717A1D10
Concentration (μg/mL)0.31.03.00.31.03.0
Dermatophagoides pteronyssinus (1 μg/mL)
 Inhibition of IL-5 (%)14.737.3*56.7***12.518.2*34.2***
 Inhibition of IL-13 (%)29.641.4***46.1***4.322.9***41.3***
Dermatophagoides pteronyssinus (3 μg/mL)
 Inhibition of IL-5 (%)17.735.9***49.3***32.039.653.3***
 Inhibition of IL-13 (%)18.734.3***40.2***32.535.4***44.3***
Der p 2 (1 μg/mL)
 Inhibition of IL-5 (%)30.351.1***79.0***16.141.1*71.0***
 Inhibition of IL-13 (%)32.148.6*78.4*20.9*50.0*77.3*
Der p 2 (3 μg/mL)
 Inhibition of IL-5 (%)46.662.6***69.7***35.443.7***86.4***
 Inhibition of IL-13 (%)43.7*63.4*84.0*43.3*58.2*81.4*
  1. Abbreviation: MoAb Monoclonal antibody, HDM House dust mite, IL Interleukin, Der p 2 D. pteronyssinus or its group 2 allergens
  2. The percent inhibition of cytokine release was calculated as follows: 100 × (cytokine level with IgG2a treatment − cytokine level with anti-Dectin-2 MoAb treatment) / (cytokine level with IgG2a treatment)
  3. Mann–Whitney U test was used to compare the percent inhibition of cytokine release between anti-Dectin-2 MoAb and IgG2a
  4. * p < 0.05; ** p < 0.01; *** p < 0.005