Antibody blockade of Dectin-2 suppresses house dust mite-induced Th2 cytokine production in dendritic cell- and monocyte-depleted peripheral blood mononuclear cell co-cultures from asthma patients

Table 3 Inhibition of IL-5 and IL-13 production in monocyte-derived dendritic cells/CD14⁻ peripheral blood mononuclear cell co-culture in HDM stimulation by anti-Dectin 2 monoclonal antibody in patients with asthma

Anti-Dectin-2 MoAb	6A4G7			17A1D10
Concentration (μg/mL)	0.3	1.0	3.0	0.3	1.0	3.0
Dermatophagoides pteronyssinus (1 μg/mL)
Inhibition of IL-5 (%)	14.7	37.3^*	56.7^***	12.5	18.2^*	34.2^***
Inhibition of IL-13 (%)	29.6	41.4^***	46.1^***	4.3	22.9^***	41.3^***
Dermatophagoides pteronyssinus (3 μg/mL)
Inhibition of IL-5 (%)	17.7	35.9^***	49.3^***	32.0	39.6	53.3^***
Inhibition of IL-13 (%)	18.7	34.3^***	40.2^***	32.5	35.4^***	44.3^***
Der p 2 (1 μg/mL)
Inhibition of IL-5 (%)	30.3	51.1^***	79.0^***	16.1	41.1^*	71.0^***
Inhibition of IL-13 (%)	32.1	48.6^*	78.4^*	20.9^*	50.0^*	77.3^*
Der p 2 (3 μg/mL)
Inhibition of IL-5 (%)	46.6	62.6^***	69.7^***	35.4	43.7^***	86.4^***
Inhibition of IL-13 (%)	43.7^*	63.4^*	84.0^*	43.3^*	58.2^*	81.4^*

Abbreviation: MoAb Monoclonal antibody, HDM House dust mite, IL Interleukin, Der p 2 D. pteronyssinus or its group 2 allergens
The percent inhibition of cytokine release was calculated as follows: 100 × (cytokine level with IgG2a treatment − cytokine level with anti-Dectin-2 MoAb treatment) / (cytokine level with IgG2a treatment)
Mann–Whitney U test was used to compare the percent inhibition of cytokine release between anti-Dectin-2 MoAb and IgG2a
* p < 0.05; ** p < 0.01; *** p < 0.005

ISSN: 1423-0127