Fig. 2

The morphology and growth kinetics of fallopian tube epithelial stem cells (FTEC). (a, b) After dissociation and culture, FTEC showed polygonal morphology at P2 (a) and P6 (b). Scale bar 100 μm. (c) After culturing for 7 days, the growth kinetics of FTEC using XTT assay was evaluated on day 3 and day 7. (d) Cumulative population doubling of FTEC. Population doubling was measured at each passage. Data are expressed as the mean ± SD (n = 3). (e-h) Flow cytometry analysis for the expression of cancer stem cell markers on FTEC and HGSOC cell lines. CD24, CD44, CD117, ROR1, and CD133 were evaluated in low (P2, e), high passages (P8, f) of FTEC, and in KURAMOCHI (g) and OVSAHO (h) cells