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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Human fallopian tube epithelial cells exhibit stemness features, self-renewal capacity, and Wnt-related organoid formation

Fig. 5

FTEC formed spheres had structures, markers, and stem cell markers similar to those in the tubal epithelium. (a) FTEC cultured in a low-attachment dish in serum replacement medium from Day 0 to Day 14. The increasing size was noted at 7 days and 14 days. (b-e) Confocal immunofluorescence image of tubal epithelial cell markers including detyrosinated TUBULIN and CDH1 (an epithelial cell marker), PAX8 (red) and DAPI (blue, nuclei), vimentin (red) and another ciliated cell marker FOXJ1 (green). Scale bar = 50 μm. (e) The FTEC spheroids revealed positive IHC for LGR5. DAPI (blue) staining represented cell nuclei. Scale bar = 50 μm

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