Fig. 9

FTEC generated organoid-like mass after cocultured with human umbilical vein endothelial cells (HUVEC) and fallopian tube mesenchymal stem cells (FTMSC) and could be inhibited by DKK1. (a) Self-organization of three-dimensional FTEC co-cultured with HUVECs and FTMSCs on day 1, day 4, and day 7. (b) After adding DKK1 (Wnt signal inhibitor), no organoid was formed after 7 days. Scale bar = 100 μm. (c) The formed organoid positive staining for CD31 (endothelial marker) and PAX8 (secretory cell marker). Scale bar = 50 μm. The qRT-PCR showed that organoid with treating DKK1 appeared to have no influence on the expression of PAX8 (d) and a decrease in expressions of FOXJ1 (e) and LGR5 (f). *p < 0.05, **p < 0.01