Fig. 10

The effect of σ^E activation on the motility and transcript level of flhD in the prc⁺ ExPEC strain. (a) Motility diameters of the indicated strains. (b) Relative mRNA levels of flhD and yiiS determined by qPCR. The yiiS gene served as the reporter of σ^E activation. The mRNA level of each gene in a strain was normalized to the ftsZ level and presented as a relative level compared to that in WT-RS218 harboring pBAD (WT-RS218/pBAD). Arabinose (0.2%) was used to induce the expression of RseA that was encoded in the pRseA plasmid (Table 1) and driven by the arabinose-inducible promoter on the plasmid. The results were derived from experiments performed in triplicate and are shown as the means ± standard deviations. pRseA, pBAD harboring rseA