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Fig. 10 | Journal of Biomedical Science

Fig. 10

From: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli

Fig. 10

The effect of σE activation on the motility and transcript level of flhD in the prc+ ExPEC strain. (a) Motility diameters of the indicated strains. (b) Relative mRNA levels of flhD and yiiS determined by qPCR. The yiiS gene served as the reporter of σE activation. The mRNA level of each gene in a strain was normalized to the ftsZ level and presented as a relative level compared to that in WT-RS218 harboring pBAD (WT-RS218/pBAD). Arabinose (0.2%) was used to induce the expression of RseA that was encoded in the pRseA plasmid (Table 1) and driven by the arabinose-inducible promoter on the plasmid. The results were derived from experiments performed in triplicate and are shown as the means ± standard deviations. pRseA, pBAD harboring rseA

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