Fig. 3

The levels of FliC expression and motility of RS218, CFT073, UTI89, and their prc mutants. The effects of prc deletion on FliC expression and motility in the ExPEC strains RS218 (a), CFT073 (b), and UTI89 (c). The results of western blot analyses of the total cell lysates and the OM fractions of the indicated bacterial strains are shown in the left panels of (a), (b), and (c). OmpA served as a loading control. The motilities of the indicated strains on 0.3% agar plates are shown in the right panels of (a), (b), and (c). pCL1920, the empty plasmid vector; pPrc, the plasmid pCL1920 harboring prc (Table 1). The asterisk indicates the FliC protein in the total lysate of CFT073