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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli

Fig. 5

Effects of increased FlhDC expression on the FliC level and motility in the prc mutant. FliC and HA-tagged FlhD levels (a) and the motility (b) of ∆prc-RS218/pBAD-FlhDC with different levels of L-arabinose treatment. Each motility quantitative result was derived from experiments performed in triplicate and is presented as the means ± standard deviations. The plasmid pBAD-FlhDC harbored HA-tagged flhD and His-tagged flhC genes that were under the control of an arabinose-inducible promoter (Table 1). The levels of FliC, FlhD, and OmpA were determined by western blot analyses with a rabbit anti-FliC antiserum and an anti-HA antibody and mouse anti-OmpA antiserum, respectively. The levels of OmpA served as loading controls

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