Fig. 9
The effects of σE and RcsCDB inactivation on the transcript levels of the flhDC operon and the FliC levels in the prc mutant. (a and b) The mRNA levels of flhD, yiiS, and yjbE in the indicated strains determined by qPCR. The levels of yiiS and yjbE reflect the activation levels of the σE and RcsCDB systems, respectively. The mRNA level of each gene in a strain was normalized to the ftsZ level and presented as a relative level compared to that in WT-RS218. The results were derived from experiments performed in triplicate and are shown as the means ± standard deviations. (c and d) The levels of FliC in the indicated strains were determined by western blot analysis with a rabbit anti-FliC antiserum. OmpA levels served as loading controls