Strains or plasmids | Relevant information | Reference |
---|---|---|
Strains | ||
WT-RS218 | RS218 isolated from the cerebrospinal fluid of a neonate with meningitis | [4] |
Δprc-RS218 | RS218 with a prc deletion | [4] |
ΔlacZ-RS218 | RS218 with a lacZ deletion | This study |
ΔlacZΔprc-RS218 | RS218 with lacZ and prc deletions | This study |
ΔrseA-RS218 | RS218 with a rseA deletion | This study |
ΔdegSΔprc-RS218 | RS218 with degS and prc deletions | This study |
lacZ::degSΔdegSΔprc-RS218 | ΔdegSΔprc-RS218 with complementary degS at the lacZ gene chromosomal locus | This study |
ΔrcsBΔprc-RS218 | RS218 with rcsB and prc deletions | This study |
lacZ::rcsBΔrcsBΔprc-RS218 | ΔrcsBΔprc-RS218 with complementary rcsB at the lacZ gene chromosomal locus | This study |
ΔdegSΔrcsBΔprc-RS218 | RS218 with degS, rcsB and prc deletions | This study |
ΔompRΔprc-RS218 | RS218 with ompR and prc deletions | This study |
ΔqseBΔprc-RS218 | RS218 with qseB and prc deletions | This study |
ΔcpxRΔprc-RS218 | RS218 with cpxR and prc deletions | This study |
Spr-3xFlag-RS218 | RS218 with spr-3xFlag tag at the spr gene chromosomal locus | This study |
Spr-3xFlag-Δprc-RS218 | RS218 with spr-3xFlag tag and prc deletion at the spr gene chromosomal locus | This study |
ΔsprΔprc-RS218 | RS218 with spr and prc deletions | This study |
ΔfliC-RS218 | RS218 with a fliC deletion | This study |
WT-CFT073 | CFT073 isolated from the blood and urine of a woman with acute pyelonephritis | [39] |
Δprc-CFT073 | CFT073 with a prc deletion | This study |
ΔlacZ-CFT073 | CFT073 with a lacZ deletion | This study |
ΔlacZΔprc-CFT073 | CFT073 with lacZ and prc deletions | This study |
ΔdegSΔprc-CFT073 | CFT073 with degS and prc deletions | This study |
ΔrcsBΔprc-CFT073 | CFT073 with rcsB and prc deletions | This study |
ΔfliC-CFT073 | CFT073 with a fliC deletion | This study |
WT-UTI89 | UTI89 isolated from the urine of a patient with cystitis | [40] |
Δprc-UTI89 | UTI89 with a prc deletion | This study |
ΔlacZ-UTI89 | UTI89 with a lacZ deletion | This study |
ΔlacZΔprc-UTI89 | UTI89 with lacZ and prc deletions | This study |
ΔfliC-UTI89 | UTI89 with a fliC deletion | This study |
Plasmids | ||
pCL1920 | Low copy-number plasmid | [41] |
pPrc | pCL1920 harboring the prc gene that is under control of the lac promoter | [4] |
pPrc-S430A | pCL1920 harboring the prc gene containing a serine point mutation resulting in inactivation of the serine protease | This study |
pPrc-K455A | pCL1920 harboring the prc gene containing a lysine point mutation resulting in inactivation of the serine protease | This study |
pUC19 | Expression plasmid containing the lac promoter | NEB |
pFlhDC | pUC19 harboring an HA-tagged flhD and a His-tagged flhC gene that are under control of the lac promoter | This study |
pACYC184 | Expression plasmid containing constitutively expressed Tetr and Cmr genes | NEB |
pDegQ | pACYC184 harboring the degQ gene that is under control of chloramphenicol resistant gene’s promoter | This study |
pBAD | Expression plasmid containing an arabinose-inducible promoter | Invitrogen |
pBAD-FlhDC | pBAD harboring a HA-tagged flhD and a His-tagged flhC gene that are under control of an arabinose-inducible promoter | This study |
pRcsB | pBAD harboring the rcsB gene that is under control of an arabinose-inducible promoter | This study |
pRseA | pBAD harboring the HA-tagged-rseA gene-His-tagged that is under control of an arabinose-inducible promoter | This study |
pSpr | pBAD harboring a Flag-tagged spr gene that is under control of an arabinose-inducible promoter | This study |