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Table 1 E. coli strains and plasmids used in this study

From: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli

Strains or plasmidsRelevant informationReference
Strains
WT-RS218RS218 isolated from the cerebrospinal fluid of a neonate with meningitis[4]
Δprc-RS218RS218 with a prc deletion[4]
ΔlacZ-RS218RS218 with a lacZ deletionThis study
ΔlacZΔprc-RS218RS218 with lacZ and prc deletionsThis study
ΔrseA-RS218RS218 with a rseA deletionThis study
ΔdegSΔprc-RS218RS218 with degS and prc deletionsThis study
lacZ::degSΔdegSΔprc-RS218ΔdegSΔprc-RS218 with complementary degS at the lacZ gene chromosomal locusThis study
ΔrcsBΔprc-RS218RS218 with rcsB and prc deletionsThis study
lacZ::rcsBΔrcsBΔprc-RS218ΔrcsBΔprc-RS218 with complementary rcsB at the lacZ gene chromosomal locusThis study
ΔdegSΔrcsBΔprc-RS218RS218 with degS, rcsB and prc deletionsThis study
ΔompRΔprc-RS218RS218 with ompR and prc deletionsThis study
ΔqseBΔprc-RS218RS218 with qseB and prc deletionsThis study
ΔcpxRΔprc-RS218RS218 with cpxR and prc deletionsThis study
Spr-3xFlag-RS218RS218 with spr-3xFlag tag at the spr gene chromosomal locusThis study
Spr-3xFlag-Δprc-RS218RS218 with spr-3xFlag tag and prc deletion at the spr gene chromosomal locusThis study
ΔsprΔprc-RS218RS218 with spr and prc deletionsThis study
ΔfliC-RS218RS218 with a fliC deletionThis study
WT-CFT073CFT073 isolated from the blood and urine of a woman with acute pyelonephritis[39]
Δprc-CFT073CFT073 with a prc deletionThis study
ΔlacZ-CFT073CFT073 with a lacZ deletionThis study
ΔlacZΔprc-CFT073CFT073 with lacZ and prc deletionsThis study
ΔdegSΔprc-CFT073CFT073 with degS and prc deletionsThis study
ΔrcsBΔprc-CFT073CFT073 with rcsB and prc deletionsThis study
ΔfliC-CFT073CFT073 with a fliC deletionThis study
WT-UTI89UTI89 isolated from the urine of a patient with cystitis[40]
Δprc-UTI89UTI89 with a prc deletionThis study
ΔlacZ-UTI89UTI89 with a lacZ deletionThis study
ΔlacZΔprc-UTI89UTI89 with lacZ and prc deletionsThis study
ΔfliC-UTI89UTI89 with a fliC deletionThis study
Plasmids
pCL1920Low copy-number plasmid[41]
pPrcpCL1920 harboring the prc gene that is under control of the lac promoter[4]
pPrc-S430ApCL1920 harboring the prc gene containing a serine point mutation resulting in inactivation of the serine proteaseThis study
pPrc-K455ApCL1920 harboring the prc gene containing a lysine point mutation resulting in inactivation of the serine proteaseThis study
pUC19Expression plasmid containing the lac promoterNEB
pFlhDCpUC19 harboring an HA-tagged flhD and a His-tagged flhC gene that are under control of the lac promoterThis study
pACYC184Expression plasmid containing constitutively expressed Tetr and Cmr genesNEB
pDegQpACYC184 harboring the degQ gene that is under control of chloramphenicol resistant gene’s promoterThis study
pBADExpression plasmid containing an arabinose-inducible promoterInvitrogen
pBAD-FlhDCpBAD harboring a HA-tagged flhD and a His-tagged flhC gene that are under control of an arabinose-inducible promoterThis study
pRcsBpBAD harboring the rcsB gene that is under control of an arabinose-inducible promoterThis study
pRseApBAD harboring the HA-tagged-rseA gene-His-tagged that is under control of an arabinose-inducible promoterThis study
pSprpBAD harboring a Flag-tagged spr gene that is under control of an arabinose-inducible promoterThis study