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Table 1 E. coli strains and plasmids used in this study

From: The role of the bacterial protease Prc in the uropathogenesis of extraintestinal pathogenic Escherichia coli

Strains or plasmids

Relevant information

Reference

Strains

WT-RS218

RS218 isolated from the cerebrospinal fluid of a neonate with meningitis

[4]

Δprc-RS218

RS218 with a prc deletion

[4]

ΔlacZ-RS218

RS218 with a lacZ deletion

This study

ΔlacZΔprc-RS218

RS218 with lacZ and prc deletions

This study

ΔrseA-RS218

RS218 with a rseA deletion

This study

ΔdegSΔprc-RS218

RS218 with degS and prc deletions

This study

lacZ::degSΔdegSΔprc-RS218

ΔdegSΔprc-RS218 with complementary degS at the lacZ gene chromosomal locus

This study

ΔrcsBΔprc-RS218

RS218 with rcsB and prc deletions

This study

lacZ::rcsBΔrcsBΔprc-RS218

ΔrcsBΔprc-RS218 with complementary rcsB at the lacZ gene chromosomal locus

This study

ΔdegSΔrcsBΔprc-RS218

RS218 with degS, rcsB and prc deletions

This study

ΔompRΔprc-RS218

RS218 with ompR and prc deletions

This study

ΔqseBΔprc-RS218

RS218 with qseB and prc deletions

This study

ΔcpxRΔprc-RS218

RS218 with cpxR and prc deletions

This study

Spr-3xFlag-RS218

RS218 with spr-3xFlag tag at the spr gene chromosomal locus

This study

Spr-3xFlag-Δprc-RS218

RS218 with spr-3xFlag tag and prc deletion at the spr gene chromosomal locus

This study

ΔsprΔprc-RS218

RS218 with spr and prc deletions

This study

ΔfliC-RS218

RS218 with a fliC deletion

This study

WT-CFT073

CFT073 isolated from the blood and urine of a woman with acute pyelonephritis

[39]

Δprc-CFT073

CFT073 with a prc deletion

This study

ΔlacZ-CFT073

CFT073 with a lacZ deletion

This study

ΔlacZΔprc-CFT073

CFT073 with lacZ and prc deletions

This study

ΔdegSΔprc-CFT073

CFT073 with degS and prc deletions

This study

ΔrcsBΔprc-CFT073

CFT073 with rcsB and prc deletions

This study

ΔfliC-CFT073

CFT073 with a fliC deletion

This study

WT-UTI89

UTI89 isolated from the urine of a patient with cystitis

[40]

Δprc-UTI89

UTI89 with a prc deletion

This study

ΔlacZ-UTI89

UTI89 with a lacZ deletion

This study

ΔlacZΔprc-UTI89

UTI89 with lacZ and prc deletions

This study

ΔfliC-UTI89

UTI89 with a fliC deletion

This study

Plasmids

pCL1920

Low copy-number plasmid

[41]

pPrc

pCL1920 harboring the prc gene that is under control of the lac promoter

[4]

pPrc-S430A

pCL1920 harboring the prc gene containing a serine point mutation resulting in inactivation of the serine protease

This study

pPrc-K455A

pCL1920 harboring the prc gene containing a lysine point mutation resulting in inactivation of the serine protease

This study

pUC19

Expression plasmid containing the lac promoter

NEB

pFlhDC

pUC19 harboring an HA-tagged flhD and a His-tagged flhC gene that are under control of the lac promoter

This study

pACYC184

Expression plasmid containing constitutively expressed Tetr and Cmr genes

NEB

pDegQ

pACYC184 harboring the degQ gene that is under control of chloramphenicol resistant gene’s promoter

This study

pBAD

Expression plasmid containing an arabinose-inducible promoter

Invitrogen

pBAD-FlhDC

pBAD harboring a HA-tagged flhD and a His-tagged flhC gene that are under control of an arabinose-inducible promoter

This study

pRcsB

pBAD harboring the rcsB gene that is under control of an arabinose-inducible promoter

This study

pRseA

pBAD harboring the HA-tagged-rseA gene-His-tagged that is under control of an arabinose-inducible promoter

This study

pSpr

pBAD harboring a Flag-tagged spr gene that is under control of an arabinose-inducible promoter

This study