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Table 1 Plasmids and bacterial strains used in this study

From: Melatonin restores Muc2 depletion induced by V. vulnificus VvpM via melatonin receptor 2 coupling with Gαq

Strain or plasmidRelevant characteristics aReference or source
Bacterial strains
V. vulnificus
  M06–24/OClinical isolate; virulent; WTLaboratory collection
  ML05MO6–24/O ΔVvpM; Kmr; VvpM mutLee et al., 2013 [22]
E. coli
  DH5αλ ϕ80dlacZΔM15 Δ (lacZYA-argF)U169 recA1 endA1 hsdR17 (rK mK) supE44 thi-1 gyrA relA1; plasmid replicationLaboratory collection
  S17–1λpirλ-pir lysogen; thi pro hsdR hsdM+ recA RP4–2 Tc::Mu-Km::Tn7;Tpr Smr; host for π-requiring plasmids; conjugal donorSimon et al., 1983 [23]
Plasmids
 pDM4R6K γ ori sacB; suicide vector; oriT of RP4; CmrMilton et al., 1996 [24]
 pRK415IncP ori, broad-host-range vector; oriT of RP4; TcrKeen et al., 1988 [25]
 pKK1535pRK415 with VvpM; TcrThis study
  1. a Kmr, kanamycin resistant; Tpr, trimethoprim resistant; Smr, streptomycin resistant; Cmr, chloramphenicol resistant; Tcr, tetracycline resistant