Fig. 6
Weight, temperature change and viral shedding in nasal wash of ferrets. Ferrets were infected intranasally with 2.5 × 105 plaque-forming units (PFU) in 500 μL of viruses with NP substitutions. a Temperature and (b) weight changes were monitored. Nasal wash specimens were collected and expressed as 50% log10 tissue culture infectious doses (TCID50). In (c) viral shedding curve and (d) viral shedding dot plot, viruses with different substitutions were separated by colors and shapes; each dot represents the viral titer in nasal wash of each ferret. Two-way ANOVA, multiple comparisons with Bonferroni post-hoc test was performed; *p < 0.05, **p < 0.01, ***p < 0.001