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Fig. 5 | Journal of Biomedical Science

Fig. 5

From: Treatment of rats with spinal cord injury using human bone marrow-derived stromal cells prepared by negative selection

Fig. 5

Astrogliosis was not affected by bmSC and MP treatment. Evaluation of GFAP-IR in spinal cord tissue ten weeks after SCI. a-b Overview of scar formation around the lesion center in a typical example; nuclear labeling with Hoechst-33342 (a) was combined with GFAP (b) immunostaining; 5x objective, scale bar 0.5 mm in a. c Reactive astrocytes in the gey matter outside of the lesion center. d-f Higher magnification of GFAP-IR close to the lesion site in SCI rats with control treatment (d), MP injections (e), bmSC implants (f), and g in the white matter of an animal without SCI; 20x objective, images d-g with the same times of exposure, scale bar 100 μm in g. h Quantification of GFAP-IR (integrated density) near the lesion site revealed no significant differences between SCI treatment groups (t-tests, p > 0.5). Data were normalized to GFAP-IR in the white matter of rats without lesion (statistical difference not indicated); bars show means and SEM, n = 5–6 rats/group

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