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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Chemoresistant ovarian cancer enhances its migration abilities by increasing store-operated Ca2+ entry-mediated turnover of focal adhesions

Fig. 3

Chemoresistant IGROV1 sublines enhance dynamics of focal adhesions. a-b FAK, paxillin, vinculin, talin, phosphorylated FAK (pTyr397-FAK), and phosphorylated paxillin (pTyr118-Paxillin) were detected using immunoblotting in IGROV1 parental (WT) and chemoresistant (CP, SRT) cells. β-actin served as the internal control. c-d Real-time recordings by TIRFM was used to evaluate the turnover of EGFP-tagged focal adhesion molecules (FAK, paxillin, vinculin, talin) in IGROV1 cells. Quantitative analysis of (c) assembly and (d) disassembly rates of focal adhesion molecules in IGROV1 cells. Each bar represents mean ± SEM from at least 15 cells. *: significant difference between chemoresistant (CP, SRT) and parental (WT) cells. *: p < 0.05; **: p < 0.01; ***: p < 0.001 by Student’s t-test

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