Fig. 6

SOCE inhibitors decrease migration ability of chemoresistant IGROV1 cells in transwell assay. In vitro transwell migration assay was performed to evaluate the effect of SOCE inhibitors (2 μΜ SKF-96365, 10 μΜ YM-58483, 0.1 μM 2-APB) on chemoresistant IGROV1 (CP, SRT) cells. a, c Representative photomicrographs of (a) IGROV1-CP and (c) IGROV1-SRT cells that penetrated a filter of pore size of 8 μm. Scale bars, 200 μm. b, d Migrated (b) IGROV1-CP and (d) IGROV1-SRT cells were counted in 15 random fields on the lower surface of the filters and expressed as percentage (%) of SOCE inhibitor pretreated cells compared with DMSO control (Ctrl). Each bar represents mean ± SEM from three independent experiments. *: significant difference between cells treated with SOCE inhibitors and DMSO control (Ctrl). *: p < 0.05; **: p < 0.01; ***: p < 0.001 by Student’s t-test