Fig. 8

Calpain inhibitors reduce cell migration in chemoresistant IGROV1 cells. The calpain inhibitors were applied to examine their effects on cell migration by wound healing assay. Chemoresistant IGROV1 cells (CP, SRT) were seeded into silicon inserts with 10% FBS medium. Following cell adhesion, cells under pretreatment with calpain inhibitors for 30 min, including 50 μM PD105606, 50 μM PD151746, 10 μM ALLN, 10 μM calpeptin, and 50 μM calpastatin. Non-calpain inhibitor pretreated cells were treated with DMSO. Then, inserts were removed and incubated cells in medium for 36 h. a Phase images were captured at 36 h and wound spaces were analyzed using ImageJ. b Cellular migratory ability is presented as the percentage of wound closure. Each bar represents mean ± SEM from three independent experiments. *: significant difference between calpain inhibitor pretreated cells and non-calpain inhibitor pretreated cells. **: p < 0.01; ***: p < 0.001 by Student’s t-test