Fig. 9
Ca2+ signaling does not affect the activity of focal adhesion molecules and the expression of SOCE components. a Pretreatment of SOCE inhibitors (2 μΜ SKF-96365, 10 μΜ YM-58483, 0.1 μM 2-APB) on IGROV1 parental (WT) and chemoresistant (CP, SRT) IGROV1 cells for 24 h. FAK, paxillin, phosphorylated FAK (pTyr397-FAK), and phosphorylated paxillin (pTyr118-Paxillin) were detected using immunoblotting. β-actin served as the internal control. b The intracellular Ca2+ chelator, BAPTA-AM (1 μM), was used to deplete intracellular Ca2+. IGROV1 cells were cultured in medium with (2 mM) or without (0 mM) Ca2+ for 48 h. After collection of protein lysates, STIM1, TRPC1, pTyr118-Paxinllin and paxillin were detected using immunoblotting in IGROV1 parental (WT) and chemoresistant (CP, SRT) cells. β-actin served as the internal control