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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Clinical-grade cryopreserved doxorubicin-loaded platelets: role of cancer cells and platelet extracellular vesicles activation loop

Fig. 2

Characterization of fresh and cryopreserved DOX-loaded PLT. (a) Scheme of preparation of PD and FPD. (b) Fluorescent images of FPD compared to PD. (DOX: red, PLT membrane marker: green) (c) Western blot analysis showing similar expression of membrane surface markers: CD41, CD61, and PAR-1 in PLT, DOX-loaded PLT, cryopreserved PLT and cryopreserved DOX-loaded PLT; GAPDH was used as a control. The PLT functional assay of R time (d) and MA values (e) of PLT, DOX-loaded PLT, cryopreserved PLT, cryopreserved DOX-loaded PLT, and platelet-poor plasma (PPP) determined by PLT functional thromboelastography assay. * p < 0.05, ** p < 0.01, ****p < 0.0001, ns = non-significant. Abbreviations: PC: platelet concentrate, PLT: platelet, DOX: doxorubicin, PAS: PLT additive solution, PD: DOX-loaded PLT, FPD: cryopreserved DOX-loaded PLT

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Journal of Biomedical Science

ISSN: 1423-0127