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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3—PBX1 and β-catenin—ABCG2 signaling

Fig. 2

Colocalization of c-Kit and PHB is positively correlated in the membrane raft domain of ovarian serous carcinoma. a Human ovarian tissue sections were stained using rhodamine-tagged CKGGRAKDC (red) for PHB, Alexa Fluor 488 (green) for c-Kit and DAPI (blue) for nuclei to detect colocalization of PHB and c-Kit by confocal fluorescence microscopy. The rhodamine-tagged CKGGRAKDC peptide is known to bind PHB in adipose endothelial cells. Scale bar = 10 μm. b, c and d The graph indicates the levels of plasma membrane-associated PHB and c-Kit at different stages of ovarian serous carcinoma (Normal: n = 10; Stage I: n = 32; Stage II: n = 14; Stage III + IV: n = 18). Ipm: fluorescence intensity at the plasma membrane; Icyt: fluorescence intensity at the cytoplasm. Data were analyzed using one-way ANOVA followed by Dunnett’s test; horizontal bars indicate the means ± SEM. e Scatter plot shows the correlation of c-Kit with PHB. R: Pearson correlation coefficients; R2 means ‘the goodness of fit’. Statistical significance was calculated by Pearson correlation coefficients. ****, P < 0.0001

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