Fig. 7
Phospho-PHBY259 in the membrane raft promotes ovarian cancer stem cell survival and potentiates drug-resistant phenotypes. a SKOV3_c-Kit and SKOV3GL-G4 cells were transfected with empty vector or mutant PHB plasmid (pD-PHBY259F-HA) for 48 h and plated at a density of 300 cells/well in 6-well plates for 14 days. The colony formation was visualized with crystal violet. b Spheroid-forming assay in SKOV3_c-Kit (or SKOV3GL-G4) cells transfected with empty vector or pD-PHBY259F-HA plasmid. Representative photos were taken on days 4, 8, and 12. Spheroid sizes were digitally determined. c Carboplatin sensitivity assay. SKOV3 cells were transfected with empty vector or wild-type pD-PHB-HA for 48 h. Cells were treated with carboplatin for 72 h, and cell viability was detected. d SKOV3_c-Kit and SKOV3GL-G4 cells were transfected with empty vector or pD-PHBY259F-HA plasmid. After treatment with carboplatin for 72 h, cell viability was measured using the CCK-8 Assay Kit. The above data represent the mean ± SD of three independent experiments. ***, P < 0.001; **, P < 0.01; *, P < 0.05, t test