Fig. 10

Knockdown of PHB1/2 in DENV-3 infected cells. The efficiency of siRNA knockdown in reverse transfected A SH-SY5Y cells with siPHB1_ (A/B/C), B SH-SY5Y cells with siPHB2_ (A/B/C) and C U-87 MG cells with siPHB2_ (A/B/C) for 48 h was verified by Western blot. Non-targeting siRNA served as control. At 48 h post transfection, cells were infected with DENV-3 (MOI 10). Virus titre in culture supernatant of infected D-i SH-SY5Y, E-i CHME-3 and F-i U-87 MG cells was determination by plaque assay at 30 h PI. Data are presented as mean ± SD from three independent experiments. Statistical analysis was performed using one-way ANOVA with bonferroni post test (ns p > 0.5, ** p < 0.001, *** p < 0.0001). Cell lysates were harvested from infected (D-ii) SH-SY5Y, (E-ii) CHME-3 and (F-ii) U-87 MG cells for detection of the level of PHB1/2 protein expression by Western blot analysis, using β-actin as an internal control