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Fig. 7 | Journal of Biomedical Science

Fig. 7

From: Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

Fig. 7

Western Blot of Co-IP demonstrates the interaction between PHB1/2 and DENV-3 E proteins on SH-SY5Y/ CHME-3 cell membrane. A The presence of DENV-3 E protein (45‚ÄČkDa) in the complex (SH-SY5Y membrane + DENV-3 incubated with anti PHB2 antibody was confirmed (arrow) by Western blot analysis using anti-DENV-3 antibody (L1). B The presence of PHB1/2 protein in the complex (SH-SY5Y membrane + DENV-3 incubated with anti DENV antibody was confirmed (arrow) by Western blot analysis using anti-PHB1/2 antibody (L2). C The presence of DENV-3 E protein and PHB1 (30‚ÄČkDa) protein in the complex (CHME-3 membrane + DENV-3 incubated with anti-DENV antibody) was confirmed (arrow) by Western blot analysis using anti-PHB1 and anti-DENV-3 antibodies (L3). D The presence of PHB1/2 protein and DENV-3 E protein in the complex (CHME-3 membrane + DENV-3 incubated with anti-DENV antibody) was confirmed (arrow) by Western blot analysis using anti-PHB1/2 and anti-DENV antibody (L4). Lane M represents the molecular weight of protein marker. Lane C represents the absence of DENV-3 E protein, run as a control

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