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Table 3 SEQUEST search results of LC-MS/MS analysis of DENV-3 binding protein on CHME-3 cell membrane

From: Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

AccessionGene symbolDescriptionSequest ScorePSMsUnique PeptidesMW [kDa]
A
5174447GNB2L1guanine nucleotide-binding protein subunit beta-2-like 1118.74591535.06
4506661RPL7A60S ribosomal protein L7a70.36261829.98
5803225YWHAE14–3-3 protein epsilon54.73261329.16
4507879VDAC1voltage-dependent anion-selective channel protein 143.0618930.75
94721250VAPAvesicle-associated membrane protein-associated protein A isoform 129.3010632.59
50345988ATP5C1ATP synthase subunit gamma, mitochondrial isoform L (liver) precursor28.2410832.98
296317337VDAC2voltage-dependent anion-selective channel protein 2 isoform 126.1510733.35
4502901CLTBclathrin light chain B isoform a23.83101023.17
4502709CDK1cyclin-dependent kinase 1 isoform 120.8910734.07
4502107ANXA5annexin A518.929835.91
4505581PRKRAinterferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 118.668534.38
4502317ATP6V1E1V-type proton ATPase subunit E 1 isoform a15.388526.13
221307584PHB2prohibitin-2 isoform 19.824433.28
20149675EFHD2EF-hand domain-containing protein D28.924326.68
4759302VAPBvesicle-associated membrane protein-associated protein B/C isoform 18.494327.21
16936528CDK2cyclin-dependent kinase 2 isoform 18.355433.91
208879465VDAC3voltage-dependent anion-selective channel protein 3 isoform 27.324330.77
156071459SLC25A5ADP/ATP translocase 27.203332.83
B
156071459SLC25A5ADP/ATP translocase 279.7637832.83
156071462SLC25A6ADP/ATP translocase 372.5535332.85
528281407PHBprohibitin isoform 168.55351329.79
4506727RPS4Y140S ribosomal protein S4, Y isoform 143.4018429.44
5803227YWHAQ14–3-3 protein theta42.5922927.75
4759302VAPBvesicle-associated membrane protein-associated protein B/C isoform 139.07161227.21
  1. Proteins are arranged in descending order based on scores obtained (A) ~ 31 kDa protein (B) ~ 28 kDa protein. PHB2, 33 kDa protein and PHB1 30 kDa protein was identified as a bona fide protein to serve as a DENV-3 receptor on CHME-3 cells. Bold fonts represent the proteins identified in this study for further characterization based on the results obtained in the algorithm