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Table 3 SEQUEST search results of LC-MS/MS analysis of DENV-3 binding protein on CHME-3 cell membrane

From: Prohibitin 1/2 mediates Dengue-3 entry into human neuroblastoma (SH-SY5Y) and microglia (CHME-3) cells

Accession

Gene symbol

Description

Sequest Score

PSMs

Unique Peptides

MW [kDa]

A

5174447

GNB2L1

guanine nucleotide-binding protein subunit beta-2-like 1

118.74

59

15

35.06

4506661

RPL7A

60S ribosomal protein L7a

70.36

26

18

29.98

5803225

YWHAE

14–3-3 protein epsilon

54.73

26

13

29.16

4507879

VDAC1

voltage-dependent anion-selective channel protein 1

43.06

18

9

30.75

94721250

VAPA

vesicle-associated membrane protein-associated protein A isoform 1

29.30

10

6

32.59

50345988

ATP5C1

ATP synthase subunit gamma, mitochondrial isoform L (liver) precursor

28.24

10

8

32.98

296317337

VDAC2

voltage-dependent anion-selective channel protein 2 isoform 1

26.15

10

7

33.35

4502901

CLTB

clathrin light chain B isoform a

23.83

10

10

23.17

4502709

CDK1

cyclin-dependent kinase 1 isoform 1

20.89

10

7

34.07

4502107

ANXA5

annexin A5

18.92

9

8

35.91

4505581

PRKRA

interferon-inducible double-stranded RNA-dependent protein kinase activator A isoform 1

18.66

8

5

34.38

4502317

ATP6V1E1

V-type proton ATPase subunit E 1 isoform a

15.38

8

5

26.13

221307584

PHB2

prohibitin-2 isoform 1

9.82

4

4

33.28

20149675

EFHD2

EF-hand domain-containing protein D2

8.92

4

3

26.68

4759302

VAPB

vesicle-associated membrane protein-associated protein B/C isoform 1

8.49

4

3

27.21

16936528

CDK2

cyclin-dependent kinase 2 isoform 1

8.35

5

4

33.91

208879465

VDAC3

voltage-dependent anion-selective channel protein 3 isoform 2

7.32

4

3

30.77

156071459

SLC25A5

ADP/ATP translocase 2

7.20

3

3

32.83

B

156071459

SLC25A5

ADP/ATP translocase 2

79.76

37

8

32.83

156071462

SLC25A6

ADP/ATP translocase 3

72.55

35

3

32.85

528281407

PHB

prohibitin isoform 1

68.55

35

13

29.79

4506727

RPS4Y1

40S ribosomal protein S4, Y isoform 1

43.40

18

4

29.44

5803227

YWHAQ

14–3-3 protein theta

42.59

22

9

27.75

4759302

VAPB

vesicle-associated membrane protein-associated protein B/C isoform 1

39.07

16

12

27.21

  1. Proteins are arranged in descending order based on scores obtained (A) ~ 31 kDa protein (B) ~ 28 kDa protein. PHB2, 33 kDa protein and PHB1 30 kDa protein was identified as a bona fide protein to serve as a DENV-3 receptor on CHME-3 cells. Bold fonts represent the proteins identified in this study for further characterization based on the results obtained in the algorithm