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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Functions and properties of nuclear lncRNAs—from systematically mapping the interactomes of lncRNAs

Fig. 3

Mapping RNA-chromatin interactions. In ChIRP-seq, CHART-seq, and CHIRT-seq, RNA associated chromatin complexes are crosslinked by formaldehyde or glutaraldehyde, captured by antisense oligos that target a specific lncRNA, and DNA fragments that are associated with RNA-protein complex are sequenced. For all-to-all interactions (MARGI-seq, ChAR-seq, and GRID-seq), a linker is ligated to connect DNA and RNA. DNA-RNA chimeras are sequenced. HiChIRP-seq combines Hi-C and ChIRP to identify the interacting chromatin (inter- and intra-chromatin interactions) of a specific lncRNA

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