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Fig. 3 | Journal of Biomedical Science

Fig. 3

From: Long-term expansion of directly reprogrammed keratinocyte-like cells and in vitro reconstitution of human skin

Fig. 3

Differentiation potential of BN-iKCs in vitro. a Relative mRNA expression of keratinocyte lineage markers in pKCs, BN28–6 cells, and mature keratinocytes differentiated from BN28–6 cells. Stem cell markers: KRT15 and ITGA6; basal/suprabasal markers: KRT14, KRT8, and KRT18; differentiated markers: KRT1, Involucrin, and Filaggrin. GAPDH was used as a loading control. Data represent the mean ± SEM. * P < 0.05. b Immunofluorescence analysis of keratinocyte markers (ITGA6, KRT14, KRT10, and Involucrin) in mature keratinocytes differentiated from pKCs and BN28–6 cells. Nuclei were counterstained with DAPI. Scale bars = 200 μm. c Oil red O staining of sebocytes differentiated from pKCs and BN28–6 cells. Images on the right are magnified views of the boxed areas in images on the left. Scale bars = 200 μm

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