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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Long-term expansion of directly reprogrammed keratinocyte-like cells and in vitro reconstitution of human skin

Fig. 4

BN-driven direct reprogramming of M-UCs into iKCs in serum-free conditions. a Schematic diagram of the generation of BN-iKCs. b Phase-contrast image and immunostaining of stem cell markers (KRT15, KRT14, and ITGA6) in BN-MiKCs at passage 4 selected by single-colony picking. Nuclei were counterstained with DAPI. Scale bars = 200 μm. c RT-PCR analysis of stem cell markers (Col17A1, ITGA6, KRT14, and KRT15) in pKCs, M-UCs, and BN-MiKCs. d Expression of keratinocyte markers (ITGA6, KRT14, KRT10, and Involucrin) in mature keratinocytes differentiated from BN-MiKCs. Scale bars = 200 μm. e Oil red O staining of sebocytes differentiated from BN-MiKCs. The image on the right is a magnified view of the boxed area in the central image. Scale bars = 200 μm

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