Fig. 2

Defective alveolarization and cell proliferation in developing CPEB2-KO lungs. a Representative images of H&E-stained lung sections used to measure alveolar size in CP2WT and CP2KO mice at different postnatal ages (n = 5 mice per group at each time). MLI, mean linear intercept. Scales, 200 μm. b The number of BrdU-positive (BrdU⁺) proliferating cells in CP2WT and CP2KO lungs at the denoted postnatal days. Representative images of BrdU- and DAPI-labeled pulmonary sections and quantification. Scales, 50 μm. c Apoptosis in CP2WT and CP2KO lungs at the alveolar stage, determined by number of active caspase3⁺ cells. Data are mean ± s.e.m. (n = 5 mice per group at each time), n.s. not significant,*P < 0.05, **P < 0.01, and ***P < 0.001, compared with CP2WT mice by two-way ANOVA and Bonferroni post-hoc test