Fig. 5

CPEB2 depletion reduces PDGFRα-expressing MYF progenitors in lungs at alveolar stages. a,a’ Representative images and quantified results of PDGFRα-positive (PDGFRα⁺) cells in CP2WT and CP2KO lungs at denoted ages. b Representative images of BrdU-, PDGFRα- and αSMA-immunostained lung sections. b’,b” Quantification of proliferating PDGFRα⁺ (BrdU⁺PDGFRα⁺) cells and MYF progenitors (BrdU⁺PDGFRα⁺αSMA⁺) in CP2WT and CP2KO lungs (n = 5 mice per group) at indicated times. c 3D views of 60 μm image stacks of P10 WT and KO lungs immunostained with BrdU, PDGFRα and αSMA. Arrowheads and arrows in magnified images of selected alveoli denote BrdU⁺PDGFRα⁺ and BrdU⁺PDGFRα⁺αSMA⁺ cells, respectively. Data are mean ± s.e.m., *P < 0.05, **P < 0.01 and ***P < 0.001, by two-way ANOVA and Bonferroni post-hoc test. Scales, 50 μm