Fig. 7

Hyperoxia-mimetic condition downregulates CPEB2 and PDGFRα expression in primary MYF culture. a The microarray dataset (GSE8586) containing transcriptomes of umbilical cord tissues collected from premature babies in whom bronchopulmonary dysplasia (BPD) later developed or not (Ctrl). The mRNA levels of CPEB2 and PDGFRα in these samples were retrieved. b The microarray dataset (GSE99633) containing transcriptomes of pulmonary CD146⁺ mesenchymal stromal cells (MSCs) isolated from neonatal rats exposed to 21% (Ctrl) or 95% oxygen (hyperoxia) for 10 days. The log2-transformed mRNA levels of CPEB2, PDGFRα and GAPDH in these samples were retrieved. c CP2WT and CP2KO MYF cultures were treated with 100 μM H₂O₂ for the indicated times and harvested for immunoblotting. PDGFRα and CPEB2 protein levels are expressed as relative ratio after normalization to β-actin level. Data are mean ± s.e.m. from 3 independent experiments. d Normalized CPEB2 levels in WT MYFs treated with H₂O₂ were correlated with PDGFRα levels in c by Pearson’s correlation coefficient. e Schematic model of CPEB2-controlled neonatal respiration. Besides CPEB2-downregulated choline acetyltransferase (ChAT) mRNA translation in dorsal motor nucleus of the vagus (DMNV) being important for normal bronchoconstriction, CPEB2-activated PDGFRα mRNA translation in MYF progenitors and MYFs is required for proper alveolarization. *P < 0.05, **P < 0.01 and ***P < 0.001, by Student’s t test in a,b and by one-way ANOVA in c