Fig. 2

Silencing of ADHFE1 and ALDH1A2 through DNMT3B. a Relative DNMT3B expression levels in 33 of OSCC tumors (T) compared with their own adjacent normal tissues (N). b Correlation analysis of DNMT3B and ADHFE1 or ALDH1A2 in OSCC patients (n = 33) by qRT-PCR analysis. Pearson correlation coefficients and p-values were calculated as indicated. Red, tumor part; green, normal part. c Expression level of DNMT3B by RT-PCR and Western blot (W.B.) analysis in human oral keratinocyte (HOK) and OSCC cell lines. GAPDH and α-tubulin were used as internal control, respectively. d RT-PCR analysis of ADHFE1 and ALDH1A2 protein after 5-aza-dC (5 μM) treatment for 5 days. GAPDH was used as an internal control. e RT-PCR and Western blot analysis of DNMT3B in SCC-15 and OEC-M1 cells following DNMT3B knockdown (si-DNMT3B) or non-targeting siRNA control (si-SC) for 48 h. GAPDH and α-tubulin were used as internal control, respectively. f qRT-PCR analysis of ADHFE1 and ALDH1A2 expression after DNMT3B knockdown (si-DNMT3B) compared with siRNA control (si-SC) in SCC-15 and OEC-M1 cells. All data are presented as mean ± SD; ***p < 0.001