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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: MiR-30a and miR-379 modulate retinoic acid pathway by targeting DNA methyltransferase 3B in oral cancer

Fig. 6

Effects of long-term treatment of arecoline and NNK. a Expression level of miRNAs in wildtype DOK cells and DOK cells long-term treatment with 50 μM of arecoline (Are-L) or 10 μM of NNK (NNK-L) for 12 months. The data are represented as mean ± SD; **p < 0.01; ***p < 0.001 versus wildtype DOK cells. b Western blot analysis of DNMT3B level in wildtype DOK cells (Wt), Are-L and NNK-L DOK cells after treatment with 5-aza-dC (5 μM) for 5 days. α-tubulin was used as internal control. c ChIP assay of ADHFE1 and ALDH1A2 promoter region was performed in Wt, Are-L and NNK-L DOK cells using anti-DNMT3B antibody after treatment with 5-aza-dC (5 μM) for 5 days. Mouse IgG (mIgG) antibody was used as negative control. d qRT-PCR analysis of ADHFE1 and ALDH1A2 level in Wt, Are-L and NNK-L DOK cells after treatment with 5-aza-dC (5 μM) for 5 days. GAPDH was used as internal control

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