Fig. 4

Antibody responses induced by rLZE3. C57BL/6 mice (n = 5/group) received two vaccinations with PBS, rZE3, or rLZE3 (10 μg per dose) via the subcutaneous route at a two-week interval. Serum samples were collected from vaccinated mice at the indicated time points after the first vaccination. a The titers of anti-rZE3 IgG antibodies were determined by ELISA. Serum samples were collected before vaccination (week 0) and used to determine basal levels for comparison. b The Zika virus-neutralizing capacity of the serum samples was determined by FRNT. The neutralizing antibody titer was defined as the reciprocal of the highest dilution that resulted in a 50% reduction in FFUs compared to the FFUs of control samples containing the virus alone. The data represent the mean ± SE of the mean. Statistical significance was determined using the Kruskal-Wallis test with Dunn’s multiple comparison test. *p < 0.05; **p < 0.01