Fig. 3

Notch pathway is activated in METTL3 deficient endothelial cells. a Heatmap depicting differentially expressed genes between control and METTL3 deficient endothelial cells (fold change > 1.2, p-value < 0.05). b KEGG analysis of the upregulated (upper) and downregulated (down) genes in METTL3 deficient endothelial cells. c qRT-PCR and (d) western blot analysis of the expression level of HEY2 after silencing or overexpressing of METTL3. e Representative bright-field images and statistical analysis of tube formation assay and (f) cell migration assay of control and HEY2 overexpressing cells. Data are shown as mean ± SEM of three independent experiments. P values were calculated using Student’s t-test. *, P < 0.05; **, P < 0.01; ***, P < 0.001