Fig. 3

Exogenous TGR5 agonist INT777 ameliorated brain injury at 24 h and 72 h after MCAO. Representative TTC staining indicated brain infarction at 24 h and 72 h after MCAO (a, e);quantified infarct ratio(b, f), neurological scores (c, g) and brain water content(d, h) showed that INT777 decreased infarction and neurological deficits in medium dose as well as reduced brain edema of right ischemic hemisphere at 24 h and 72 h after MCAO . High dose was only analyzed at 24 h after MCAO. n = 6 for each group. *P < 0.05 vs sham, ^#P < 0.05 vs MCAO+ vehicle. Bars represent mean ± SEM. BS indicates brain stem; CB, cerebellum; LH, left hemisphere; RH, right hemisphere