Fig. 4

INT777improved BBB permeability and TGR5 interacted with BRCA1 after MCAO. a INT777 treatment significantly reduced EB dye leakage, n = 6 per group; b INT777 alleviated the TEER decrease after OGD/R, n = 6 per group; c Double immunofluorescence staining showed that co-localization of TGR5 (red) and BRCA1 (green) was increased in penumbra 24 h after MCAO, d Triple-fluorescence staining showed that TGR5 and BRCA1colocalized in endothelial cell, n = 4 per group. Scale bar 50 μm. e Representative co-IP bands showed that interactions of TGR5 with BRCA1 occurred at 24 h after MCAO, n = 6 per group. f Expression of TGR5 and BRCA1 in total protein solution was detected by Western blot analysis and relative OD ratios were reported. n = 6 for each group. *P < 0.05 vs sham, ^#P < 0.05 vs MCAO+ vehicle. Bars represent mean ± SEM