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Table 1 Primer sequences for plasmid construction of SBE mutation of Twist promoter

From: Statin inhibits large hepatitis delta antigen-Smad3 -twist-mediated epithelial-to-mesenchymal transition and hepatitis D virus secretion

Primer

Sequence

pXP2-Twist-SBE site1-mt-forward

5′-GGAGGTATAAGAGCCTCCAATTGCGCAGCTCTCGCCCA-3’

pXP2-Twist-SBE site1-mt-reverse

5′-TGGGCGAGAGCTGCGCAATTGGAGGCTCTTATACCTCC-3’

pXP2-Twist-SBE site2-mt-forward

5′-CAGCTCTCGCCCAACTCCAGCCAACCTCGCGGGCTCTGCAG-3’

pXP2-Twist-SBE- site2-mt-reverse

5′-CTGCAGAGCCCGCGAGGTTGGCTGGAGTTGGGCGAGAGCTG-3’

  1. Boldface + underlining: mutated nucleotides for disruption of SBE for Smad3 binding
  2. WT sequence of Twist promoter containing SBE consensus sequence of CAG(AC)(CC) is shown in Fig. 1b
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Journal of Biomedical Science

ISSN: 1423-0127